摘要
目的 对比评价丹参中主要活性部位的血管舒张及心肌细胞保护作用,明确各活性部位在心肌保护作用中的主要贡献。方法 分别利用大鼠胸主动脉血管环及心肌细胞缺氧再复氧损伤(HRI)模型实验对丹参4个活性部位:总丹参酮部位(DSBW-1)、丹参总酚酸与粗糖蛋白混合部位(DSBW-2)、丹参总酚酸部位(DSBW-3)和丹参粗糖蛋白部位(DSBW-4),进行心肌保护作用研究和评价。结果 血管舒张实验结果表明,DSBW-3作用最强,血管舒张百分比为(55.67±3.09)%;心肌细胞保护实验表明,DSBW-1对心肌细胞保护作用最强,与模型组相比,对HRI细胞具有显著的保护作用。结论 丹参中丹参酮和丹参酚酸类物质均有一定的心肌保护作用,但作用途径和贡献不同,推测二者在心肌保护作用中可能具有一定的协同作用。
Abstract
OBJECTIVE To evaluate the myocardial protective effect of the major active extracts from Salvia miltiorrhiza Bunge(SM), and clarify their different contributions in myocardial protection.METHODS Using rat aortic rings and cardiomyocyte hypoxia-reoxygenation injury(HRI)model, experiments of SM about four active fractions, include the total tanshinones, the mixture of total salvianolic acids and glycoproteins, the total salvianolic acides, and the glycoproteins were evaluated on myocardial protection.RESULTS The vasodilator experimental results showed that the total salvianolic acides had the strongest effect and the percentage of vasodilatation was (55.67±3.09)%. The experimental of myocardial cells protection showed that the total tanshinones had the strongest protective effect on myocardial cells, and compared with model group, it had significant protective effect on HRI cells.CONCLUSION The tanshinones and salvianolic acids in SM have myocardial protective effect, but they have different pathways and contributions, which speculates that they may have a synergistic effect in myocardial protection.
关键词
丹参活性部位 /
血管舒张作用 /
心肌细胞保护作用
{{custom_keyword}} /
Key words
Salvia miltiorrhiza active extracts /
vasodilation /
cardioprotective effects
{{custom_keyword}} /
杜娟,杨小敏,周佳慧,许佳慧,张珊,毕跃峰.
丹参中各活性部位对心肌保护作用的对比评价[J]. 中国药学杂志, 2018, 53(9): 690-694 https://doi.org/10.11669/cpj.2018.09.006
DU Juan, YANG Xiao-min, ZHOU Jia-hui, XU Jia-hui, ZHANG Shan, BI Yue-feng.
Comparative Evaluation of Cardioprotective Effects of Various Active Extracts from Salvia miltiorrhiza Bunge[J]. Chinese Pharmaceutical Journal, 2018, 53(9): 690-694 https://doi.org/10.11669/cpj.2018.09.006
中图分类号：
R965
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] GU G G. Shennong′s Classic of Materia Medica(神农本草经) [M] . Haerbin:Harbin press, 2007:244.
[2] LAM F F, YEUNG J H, CHEUNG J H. Pharmacological evidence for clacium channel inhibition by danshen(Salvia miltiorrhiza) on rat isolated femoral artery[J] . Cardiovasc Pharmacol, 2006, 47(1):139-145.
[3] ZHANG Y C. Advances in studies on chemical constituents and clinical pharmacology of Salvia miltiorrhiza[J] . Guide China Med(中国医药指南), 2005, 3(11):214-215.
[4] WANG Y Q. Studies on the Isolation, Purification and Bioactivity of Glycoprotein from Salvia miltiorrhiza Bunge[D] . Xi′an:Shanxi Normal University, 2007.
[5] GUAN R F. Synthesis and Vasodilative Activity of Tanshinone IIA Derivatives [D] . Zhengzhou:Zhengzhou University, 2012.
[6] WANG P, GAO Y, YIN J, et al. Protective effect of liposoluble components of Salvia miltiorrhiza on oxygen and glucose deprivation injury in myocardial cells[J] . Chin J Tradit Chin Med(中华中医药杂志), 2015,30(1):38-41.
[7] LIN R Z, CHEN C, TIAN D K, et al. A comparative study of salvianolic acid A and salvianolic acid B on rat myocardial ischemia reperfusion injury[J] . Lishizhen Med Mater Med Res(时珍国医国药), 2011, 22(2):412-414.
[8] KAMINSKI K, BONDA T, WOJTKOWSKA I. Oxidative stress and antioxidative defense parameters early after reperfusion therapy for acute myocardial infarction[J] . Acute Card Care, 2008, 10(2):121-126.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家自然科学基金面上项目资助(81273392);河南省科技攻关计划项目资助(162102310128)
{{custom_fund}}
PDF(1630 KB)
